Science at the Edge

 

September 29^th 2017

1400 Biomedical Physical Sciences

11:30 am

 

 

James Bardwell, Rowena G. Matthews Collegiate Professor, University of Michigan, Howard Hughes Investigator

 

“Watching a Chaperone Fold a Protein at High Resolution”

 

Dr. Bardwell was a graduate student at the University of Wisconsin with Elizabeth Craig. He was a postdoctoral researcher with John Beckwith at Harvard University. He was a Visiting Professor and Alexander von Humboldt Fellow at the Institute of Biophysics and Physical Biochemistry, University of Regensburg, Germany, before joining the faculty at the University of Michigan.

 

Chaperone characterization in the Bardwell Laboratory

 

The Bardwell laboratory has addressed four of the central mysteries of chaperone biology: how chaperones rapidly bind to proteins, how the chaperone-substrate complex is stabilized, how a chaperone can facilitate substrate folding, and what triggers substrate release^1,2. The laboratory has also devised a novel structural biology approach that enabled us to visualize the dance of chaperone-mediated folding^1,4 demonstrating that client folding occurs while bound to the chaperone³.

1. Horowitz S, Salmon L, Koldewey P, Ahlstrom LS, Martin R, Quan S, Pavel V, Afonine PV, Bedem H, Wang L, Xu Q, Trievel RC, Brooks III CL, and Bardwell JCA (2016) Visualizing chaperone-assisted folding Nature Struct. Mol. Biol 23:691-7 PMID: 27239796
2. Philipp Koldewey, Frederick Stull, Scott Horowitz, Raoul Martin, and James C. A. Bardwell (2016) Forces Driving Chaperone Action Cell 166(2):369-79 PMID: 27293188
3. Stull F, Koldewey P, Humes JR, Radford SE, and Bardwell JCA (2016) Substrate protein folds while it is bound to the ATP-independent chaperone Spy  Nature Struct. Mol.Biol. 23: 53-8. PMID: 2661926
4. Salmon L, Ahlstrom LS, Horowitz S, Dickson A, Brooks CL 3rd, Bardwell JC. (2016) Capturing a Dynamic Chaperone-Substrate Interaction Using NMR-Informed Molecular Modeling. J Am Chem Soc. 138(31):9826-39. PMID:27415450

Bardwell publications can be found by following this link:

https://www.ncbi.nlm.nih.gov/pubmed?term=bardwell jc%5BAuthor%5D

 

 

Lerena R. Heintzelman

Department of Physics & Astronomy

Michigan State University

567 Wilson Rd. Room 3261

East Lansing, MI 48824

517-884-5513