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Please note room change for this seminar SCIENCE AT THE EDGE SEMINAR QB/GEDD Friday, October 18 at 11:30am Room 1425 Biomedical and Physical Sciences Bldg. Refreshments at 11:15 Rafael Casellas Genomics & Immunity, NIAMS NIH, Bethesda, MD The NIH Mouse Regulome Project The National Institutes of Health recently launched the NIH Mouse Regulome Project (NMRP), an initiative that seeks to characterize all gene-regulatory elements and ranscription factor binding sites in the mouse genome. This is achieved by mapping genome-wide DNA methylation, chromatin modifications that demarcate promoters and enhancers, PolII binding, transcriptome analyses, and DNAseI hypersensitivity. In contrast to the previous human ENCODE project, which assigned enhancers to cognate promoters based on proximity, the NMRP makes use of a newly developed deep-sequencing technique (ChIA-PET) that precisely maps promoter-enhancer interactions with unprecedented detail. To complement these efforts, a Genome Editing facility was created to assemble hundreds of zinc finger nucleases with the goal of deleting regulatory domains and study their activity in vivo. The seminar will highlight the applications of these technologies to mouse ES cells and B lymphocytes. Helen Geiger, Administrative Assistant Quantitative Biology Graduate Program and Gene Expression in Development and Disease Biochemistry 603 Wilson Road, Room 212 East Lansing, MI 48824 Email: [log in to unmask] Phone: 517-432-9895 QB Website: http://www.qbi.msu.edu/ GEDD Website: http://www.gedd.msu.edu/