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SCIENCE AT THE EDGE SEMINAR QB/GEDD Friday, November 5 at 11:30am Room 1400 Biomedical and Physical Sciences Bldg. Refreshments at 11:15 Gary Stormo Department of Genetics, Center for Genome Sciences Washington University Medical School, St. Louis, MO Specificity of Protein-DNA Interactions: Experimental and Computational Approaches Protein-DNA interactions are an essential component of regulating gene expression. Sequence-specific transcription factors (TFs) bind to specific regions of the genome and can affect the transcription of nearby genes. Information is rapidly accumulating, through methods such as ChIP-chip and ChIP-Seq, about the genomic binding locations of many TFs in different cell types and in different conditions. At the same time it is useful to know the intrinsic specificity of TFs, their relative binding affinities to different DNA sequences, because comparison of that information with location information can provide additional details about the regulatory network. New technologies, such as PBM, SELEX and B1H, now provide high-throughput data about TF specificity. This talk will describe briefly how data from these methods can be used to infer the intrinsic specificity of TFs and how that information can be used to predict the specificity of untested TFs, including the design of TFs with novel specificity. Helen Geiger Administrative Assistant Quantitative Biology Graduate Program/ Gene Expression in Development & Disease Michigan State University 502B Biochemistry Building East Lansing, MI 48824 Phone: (517) 432-9895 Fax: (517) 353-9334 E-mail: [log in to unmask]<mailto:[log in to unmask]> Web: http://qbmi.msu.edu<http://qbmi.msu.edu/> http://www.bch.msu.edu/GEDD/index.htm<http://www.bch.msu.edu/GEDD/MSU-TRSB-Symposium.htm>