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SCIENCE AT THE EDGE SEMINAR
QB/GEDD
Friday, November 5 at 11:30am
Room 1400 Biomedical and Physical Sciences Bldg.
Refreshments at 11:15
Gary Stormo
Department of Genetics, Center for Genome Sciences
Washington University Medical School, St. Louis, MO
Specificity of Protein-DNA Interactions:
Experimental and Computational Approaches
Protein-DNA interactions are an essential component of regulating gene expression. Sequence-specific transcription factors (TFs) bind to specific regions of the genome and can affect the transcription of nearby
genes. Information is rapidly accumulating, through methods such as ChIP-chip and ChIP-Seq, about the genomic binding locations of many TFs in different cell types and in different conditions. At the same time it is useful to know the intrinsic specificity
of TFs, their relative binding affinities to different DNA sequences, because comparison of that information with location information can provide additional details about the regulatory network. New technologies, such as PBM, SELEX and B1H, now provide high-throughput
data about TF specificity. This talk will describe briefly how data from these methods can be used to infer the intrinsic specificity of TFs and how that information can be used to predict the specificity of untested TFs, including the design of TFs with novel
specificity.
Helen Geiger
Administrative Assistant
Quantitative Biology Graduate Program/
Gene Expression in Development & Disease
Michigan State University
502B Biochemistry Building
East Lansing, MI 48824
Phone: (517) 432-9895
Fax: (517) 353-9334
Web: http://qbmi.msu.edu
http://www.bch.msu.edu/GEDD/index.htm